Subjects: Biology >> Botany >> Applied botany submitted time 2018-10-26 Cooperative journals: 《广西植物》
Abstract： Due to the advantages of scanning speed and high sensitivity, ESI-Q-TOF-MS is widely applicated to identify the chemical composition of traditional Chinese medicine. In addition, ESI-Q-TOF-MS can provide the precise mass number and possible element composition of fragmentation ions.Therefore, it can accurately evaluate the quality of salvia miltiorrhiza (Danshen) in different areas of Shaanxi province and provide the basis for quality control of Danshen. An direct injection ESI-Q-TOF-MS analysis method was developed to value the chemical composition in Danshen coming from Shangzhou, Luonan, Dali, Danfeng and Tongchuan in Shaanxi province. Through analyzing the MS abundance of water soluble and lipid soluble extracts, there is a obvious variation in chemical composition. The comparison among different abundance is benefit to select the optimum planting area of Danshen. Results show that all Danshen samples from 5 different locations have 9 kinds of lipid soluble chemical composition (Tanshinone I, Tanshinone ⅡA, Tanshinone ⅡB, Cryptotanshinone, Dihydrotanshinone, Danshenxinkun A, Danshenxinkun D, 2-Isopropyl-8 -methylphenanthrene-3,4-dione and 7-beta-hydroxy-8-13- abietadiene-11,12-dione) and 9 kinds of water soluble chemical composition (Danshensu, Caffeic acid, Ferulic acid, Rosmarinic acid, Prolithospermic acid, Lithospermic acid, Protocatechuic acid, Salvianolic acid A and Salvianolic acid B). The content of Danshensu, Lithospermic acid, Salvianolic acid B and Cryptotanshinone is higher than other chemical composition with a MS abundance of 30% at least. However, with the change of planting environment, the content of the same chemical constituents in Danshen varies greatly in different regions. Tanshinones with important biological activity more exist in Danshen coming from Shangzhou, especially the MS abundance of Tanshinone I is as high as 72.6%, which is much higher than that in other 4 regions ranging from 1.8% to 11.3%. According to the comprehensive comparison, the quality of Danshen is ranged by Shangzhou > Tongchuan > Dali> Luonan> Danfeng. The direct injection ESI-Q-TOF-MS method is not easily affected by the growth environment and extraction conditions of the medicinal materials. It provides a scientific, reliable and convenient way to evaluate the medicine quality and a new way to formulate the specifications and grades of traditional Chinese medicine. The system is not only suitable for the chemical composition of Danshen in Shaanxi province, but also easily transplanted and popularized in other Danshen planting places.
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